ABSTRACT
Purpose@#Drug resistance is one of the main causes of chemotherapy failure in patients with small cell lung cancer (SCLC), and extensive biological studies into chemotherapy drug resistance are required. @*Materials and Methods@#In this study, we performed lncRNA microarray, in vitro functional assays, in vivo models and cDNA microarray to evaluate the impact of lncRNA in SCLC chemoresistance. @*Results@#The results showed that KCNQ1OT1 expression was upregulated in SCLC tissues and was a poor prognostic factor for patients with SCLC. Knockdown of KCNQ1OT1 inhibited cell proliferation, migration, chemoresistance and promoted apoptosis of SCLC cells. Mechanistic investigation showed that KCNQ1OT1 can activate transforming growth factor-β1 mediated epithelial-to-mesenchymal transition in SCLC cells. @*Conclusion@#Taken together, our study revealed the role of KCNQ1OT1 in the progression and chemoresistance of SCLC, and suggested KCNQ1OT1 as a potential diagnostic and prognostic biomarker in SCLC clinical management.
ABSTRACT
Objective To analyze stress distributions of alveolar bone around implants with different types of implant thread shapes and thread depths during dental immediate implantation, so as to provide references for the design and selection of implants. Methods The simplified model of mandible bone block, implants and mandibular molar were established by Geomagic Studio, SolidWorks and ANSYS Workbench, and vertical and oblique loads were applied on this model, respectively. Stress distributions on implants with different thread shape and thread depth as well as alveolar bone around implants were calculated. Results Under vertical loading, the peak stresses of implants, cortical bone and cancellous bone were in the range of 120.51-129.63 MPa, 9.94-13.25 MPa and 3.92-8.01 MPa, respectively. And the stress of cortical bone around V-shaped, rectangular, buttress or reverse buttress implant remained stable in the range of 0.40-0.45 mm thread depth. Under oblique loading, the peak stresses of implants, cortical bone and cancellous bone were in the range of 220.23-286.51 MPa, 33.39-45.08 MPa, 4.96-12.5 MPa, respectively. Among the models, V-shaped, buttress, reverse buttress implant with 0.45 mm thread depth showed the minimum stress. Conclusions The V-shaped, buttress or reverse buttress implant with a thread depth of 0.45 mm, or the rectangular implant with a thread depth of 0.40 mm had better biomechanical properties.